Inject and elute injection command using Biacore 1S+ and Biacore 1K+ SPR systems

This procedure describes how the Inject and elute command is used in Biacore™ 1S+ and Biacore 1K+ instruments. The command is used to elute and collect bound analyte from the ligand on the sensor surface for downstream analysis. The Inject and elute command can thus be used to “fish out” an analyte that binds a specific target on the sensor chip from a complex sample solution, e.g., tissue and plant extracts.